pPK719 | Addgene 38149 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPK719

catalog :

38149

citations: 1

Reference
Petratou D, Gjikolaj M, Kaulich E, Schafer W, Tavernarakis N. A proton-inhibited DEG/ENaC ion channel maintains neuronal ionstasis and promotes neuronal survival under stress. iScience. 2023;26:107117 pubmed publisher

product information

Catalog Number :

38149

Product Name :

pPK719

article :

doi
id	5612
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript KSII(+)

backbone_mutation

backbone_origin

Stratagene

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

Other

recombineering

growth notes :

This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3 UTR of the Cbr-unc-119 gene. The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.

origin :

pi :

alt_names

rescue construct for unc-119(ed3) mutants

cbr-unc-119

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	unc-119
sequencing_primer_3
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CBG18291
gene	Cbr-unc-119
id	8584293

genbank_ids

mutation

name

cbr-unc-119::FRT*::galk::FRT*

shRNA_sequence

size

species

6239

Caenorhabditis elegans