This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPK605
catalog :
38148
citations: 2
Reference
Pees B, Yang W, Kloock A, Petersen C, Peters L, Fan L, et al. Effector and regulator: Diverse functions of C. elegans C-type lectin-like domain proteins. PLoS Pathog. 2021;17:e1009454 pubmed publisher
Yang W, Petersen C, Pees B, Zimmermann J, Waschina S, Dirksen P, et al. The Inducible Response of the Nematode Caenorhabditis elegans to Members of Its Natural Microbiota Across Development and Adult Life. Front Microbiol. 2019;10:1793 pubmed publisher
product information
Catalog Number :
38148
Product Name :
pPK605
article :
doi
id5612
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonen/a
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Plasmid pPK605 contains two inserts: (1) unc-119 promoter, unc-119 and unc-119 3'UTR cassette and (2) pie-1 promoter, MluI/BamHI cloning site and pie-1 3'UTR Please see plasmid map link above for more details
origin :
37
pi :
alt_names
unc-119
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterunc-119
sequencing_primer_3
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_M142.1
geneunc-119
id176519
genbank_ids
mutation
nameunc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR)
shRNA_sequence
size
species
6239
Caenorhabditis elegans
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterpie-1 promoter
sequencing_primer_3M13pUC-rev
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namepie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
shRNA_sequence
size
species
6239
Caenorhabditis elegans
tags
plasmid copy :
Insert was derived from pID3.01B (Geraldine Seydoux - Addgene plasmid 22720)
resistance markers :
1305
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA