This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPK720
catalog :
38147
citations: 1
Reference
Soloviev A, Gallagher J, Marnef A, Kuwabara P. C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain. Dev Biol. 2011;351:242-53 pubmed publisher
product information
Catalog Number :
38147
Product Name :
pPK720
article :
doi10.1016/j.ydbio.2010.12.035
id5611
pubmed_id21215260
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KSII(+)
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
The pPK700 plasmid was derived by ligating the dpy-7 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)
origin :
37
pi :
alt_names
dpy-7 promoter
ptc-3
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterdpy-7
sequencing_primer_3M13pUC-fwd
sequencing_primer_5M13pUC-rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesY110A2AL.8
geneptc-3
id173631
genbank_ids
mutation
namedpy-7p::ptc-3::gfp
shRNA_sequence
size
species
tags
location
tagEGFP
resistance markers :
1305
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA