pPK348 | Addgene 38145 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPK348

catalog :

38145

citations: 1

Reference
Soloviev A, Gallagher J, Marnef A, Kuwabara P. C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain. Dev Biol. 2011;351:242-53 pubmed publisher

product information

Catalog Number :

38145

Product Name :

pPK348

article :

doi	10.1016/j.ydbio.2010.12.035
id	5611
pubmed_id	21215260

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript KSII(+)

backbone_mutation

backbone_origin

Stratagene

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5 upstream sequence, including the putative promoter Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid.

origin :

pi :

alt_names

ptc-3

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	ptc-3
sequencing_primer_3	M13pUC-rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Y110A2AL.8
gene	ptc-3
id	173631

genbank_ids

mutation

name

ptc-3::GFP

shRNA_sequence

size

species

6239

Caenorhabditis elegans