This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-N1-MITF-D
catalog :
38133
citations: 4
Reference
Chan H, Li Q, Wang X, Liu W, Hu W, Zeng J, et al. Vitamin D3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification. Autophagy. 2022;:1-18 pubmed publisher
Pogenberg V, Ballesteros Álvarez J, Schober R, Sigvaldadóttir I, Obarska Kosinska A, Milewski M, et al. Mechanism of conditional partner selectivity in MITF/TFE family transcription factors with a conserved coiled coil stammer motif. Nucleic Acids Res. 2020;48:934-948 pubmed publisher
Moon K, Kim H, Lee D, Rao M, Levine E, Lim D, et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell. 2018;44:13-28.e3 pubmed publisher
Roczniak Ferguson A, Petit C, Froehlich F, Qian S, Ky J, Angarola B, et al. The transcription factor TFEB links mTORC1 signaling to transcriptional control of lysosome homeostasis. Sci Signal. 2012;5:ra42 pubmed publisher
product information
Catalog Number :
38133
Product Name :
pEGFP-N1-MITF-D
article :
doi10.1126/scisignal.2002790
id5599
pubmed_id22692423
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that this construct contains MITF isoform 7, sometimes referred to as MITF-D.
origin :
37
pi :
alt_names
microphthalmia transcription factor, D variant
MITF variant 7
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3CGTCGCCGTCCAGCTCGACCAG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMM8, COMMAD, MI, MITF-A, WS2, WS2A, bHLHe32
geneMITF
id4286
genbank_ids
NM_001184967.1
mutationdeleted stop codon
nameMITF-D
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
resistance markers :
1299
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA