This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-N1-MITF-M
catalog :
38131
citations: 9
Reference
Tanwar J, Sharma A, Saurav S, Shyamveer -, Jatana N, Motiani R. MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis. J Biol Chem. 2022;298:102681 pubmed publisher
Liu J, Ma P, Lai L, Villanueva A, Koenig A, Bean G, et al. Transcriptional and Immune Landscape of Cardiac Sarcoidosis. Circ Res. 2022;131:654-669 pubmed publisher
Chan H, Li Q, Wang X, Liu W, Hu W, Zeng J, et al. Vitamin D3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification. Autophagy. 2022;:1-18 pubmed publisher
Liu X, Yin M, Dong J, Mao G, Min W, Kuang Z, et al. Tubeimoside-1 induces TFEB-dependent lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting mTOR. Acta Pharm Sin B. 2021;11:3134-3149 pubmed publisher
Ballesteros Álvarez J, Dilshat R, Fock V, Möller K, Karl L, Larue L, et al. MITF and TFEB cross-regulation in melanoma cells. PLoS ONE. 2020;15:e0238546 pubmed publisher
Zhang N, Dou Y, Liu L, Zhang X, Liu X, Zeng Q, et al. SA-49, a novel aloperine derivative, induces MITF-dependent lysosomal degradation of PD-L1. EBioMedicine. 2019;40:151-162 pubmed publisher
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
Li G, Yang J, Yang C, Zhu M, Jin Y, McNutt M, et al. PTENα regulates mitophagy and maintains mitochondrial quality control. Autophagy. 2018;14:1742-1760 pubmed publisher
Roczniak Ferguson A, Petit C, Froehlich F, Qian S, Ky J, Angarola B, et al. The transcription factor TFEB links mTORC1 signaling to transcriptional control of lysosome homeostasis. Sci Signal. 2012;5:ra42 pubmed publisher
product information
Catalog Number :
38131
Product Name :
pEGFP-N1-MITF-M
article :
doi10.1126/scisignal.2002790
id5599
pubmed_id22692423
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that while MITF isoforms 4 and 5 can both be referred to as MITF-M, this construct contains MITF isoform 5.
origin :
37
pi :
alt_names
microphthalmia transcription factor, M variant
MITF variant 5
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3CGTCGCCGTCCAGCTCGACCAG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMM8, COMMAD, MI, MITF-A, WS2, WS2A, bHLHe32
geneMITF
id4286
genbank_ids
NM_198158.2
mutationdeleted stop codon
nameMITF-M
shRNA_sequence
size1247
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
resistance markers :
1299
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA