pLVTHM-shREV3-4 | Addgene 38033 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVTHM-shREV3-4

catalog :

38033

citations: 1

Reference
Knobel P, Kotov I, Felley Bosco E, Stahel R, Marti T. Inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells. Neoplasia. 2011;13:961-70 pubmed

product information

Catalog Number :

38033

Product Name :

pLVTHM-shREV3-4

article :

doi
id	5580
pubmed_id	22028621

bacterial resistance :

Ampicillin

cloning :

backbone

pLVTHM

backbone_mutation

backbone_origin

Trono Laboratory

backbone_size

11085

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb;3(2):109-16. PMID:16432520

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C.

origin :

pi :

alt_names

REV3L

cloning

clone_method	Restriction Enzyme
cloning_site_3	ClaI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	?
sequencing_primer_5	?
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	R, Rev, Rev3, Sez, Sez4
gene	Rev3l
id	19714

aliases
gene	Rev3l
id	309812

aliases	POLZ, REV3
gene	REV3L
id	5980

genbank_ids

1.NM_002912.3

mutation

name

shREV3-4

shRNA_sequence

CAAAGATGCTGCTACATTA

size

species

9606

Homo sapiens

10090

Mus musculus

10116

Rattus norvegicus

human