This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSCV-N BFRF0.5
catalog :
37932
citations: 1
Reference
Rozenblatt Rosen O, Deo R, Padi M, Adelmant G, Calderwood M, Rolland T, et al. Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Nature. 2012;487:491-5 pubmed publisher
product information
Catalog Number :
37932
Product Name :
MSCV-N BFRF0.5
article :
doi10.1038/nature11288
id5852
pubmed_id22810586
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-N FLAGHA
backbone_mutation
backbone_origin
backbone_size8162
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
origin :
37
pi :
alt_names
BFRF1A
BFRF4
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPKG
sequencing_primer_3MSCV-N seq. rev
sequencing_primer_5LXSN 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV4_BFRF1A
geneBFRF1A
id3783759
genbank_ids
YP_401728.1
mutation
nameBFRF0.5
shRNA_sequence
size405
species
H. Herpesvirus 4 (EBV)
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHA
plasmid copy :
E. Kieff lab
resistance markers :
351
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA