TagRFP-T-Rabenosyn-5 | Addgene 37537 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

TagRFP-T-Rabenosyn-5

catalog :

37537

citations: 2

Reference
Thurber A, Nelson M, Frost C, Levin M, Brackenbury W, Kaplan D. IK channel activation increases tumor growth and induces differential behavioral responses in two breast epithelial cell lines. Oncotarget. 2017;8:42382-42397 pubmed publisher
Navaroli D, Bellve K, Standley C, Lifshitz L, CARDIA J, Lambright D, et al. Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Proc Natl Acad Sci U S A. 2012;109:E471-80 pubmed publisher

product information

Catalog Number :

37537

Product Name :

TagRFP-T-Rabenosyn-5

article :

doi	10.1073/pnas.1115495109
id	5515
pubmed_id	22308388

bacterial resistance :

Kanamycin

cloning :

backbone

GFP-Rabenosyn5

backbone_mutation

TagRFP-T from Roger Tsien was used to replace the GFP in the Corvera Lab GFP-Rabenosyn5.

backbone_origin

Corvera Lab

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Designed and prepared by Deborah Leonard, Deanna Navaroli and Anil Chawla (Corvera Lab) There are a few sequence discrepancies between the full sequence and the Addgene QC sequence--these do not affect the function of the plasmid, but the XhoI site located at the 3' end of the TagRFP-T on the depositor's map is NOT present in the vector.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Rabenosyn-5, ZFYVE20
gene	RBSN
id	64145

genbank_ids

40034008

mutation

name

Rabenosyn5

shRNA_sequence

size

2354

species

9606

Homo sapiens