This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNCMV 8E6
catalog :
37460
citations: 1
Reference
Spangle J, Ghosh Choudhury N, Munger K. Activation of cap-dependent translation by mucosal human papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif. J Virol. 2012;86:7466-72 pubmed publisher
product information
Catalog Number :
37460
Product Name :
pNCMV 8E6
article :
doi10.1128/JVI.00487-12
id5488
pubmed_id22553330
bacterial resistance :
Ampicillin
cloning :
backboneCMV bam neo
backbone_mutationFlag/HA tag-containing linker added at BamHI site.
backbone_origin
backbone_size6550
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The 8E6 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)
growth strain :
Expression of HPV 8E6 in mammalian cells
origin :
37
pi :
alt_names
8E6
E6
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5Bglob_intron_F (ctggtcatcatcctgccttt)
site_3_destroyed1
site_5_destroyed
entrez_gene
genbank_ids
AAD47251.1
Uniprot: P06428
mutation
nameHPV 8E6
shRNA_sequence
size542
species
HPV
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHA
resistance markers :
351
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA