This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cre Stoplight 2.4
catalog :
37402
citations: 2
Reference
Londono R, Wenzhong W, Wang B, Tuan R, Lozito T. Cartilage and Muscle Cell Fate and Origins during Lizard Tail Regeneration. Front Bioeng Biotechnol. 2017;5:70 pubmed publisher
Yang Y, Hughes T. Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells. Biotechniques. 2001;31:1036, 1038, 1040-1 pubmed
product information
Catalog Number :
37402
Product Name :
Cre Stoplight 2.4
article :
doi10.2144/01315st03
id5462
pubmed_id11730010
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size4273
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Cre Stoplight 2.4 differs from the original Cre Stoplight described in the associated publication (Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.). See the supplemental document Cre Stoplight 2.4 above for more information. Please note that Addgene digest and NGS results suggest that the plasmid contains two mCherry ORFs in tandem. This is not expected to affect plasmid function as the second mCherry should not be expressed from the plasmid.
origin :
37
pi :
alt_names
Stoplight
cloning
clone_methodRestriction Enzyme
cloning_site_3loxp
cloning_site_5BamH1, EcoRV etc.
promoterCMV
sequencing_primer_3Amp
sequencing_primer_5CAG enhancer
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre Stoplight 2.4
shRNA_sequence
size2023
species
tags
locationN terminal on insert
tagZsGreen1
locationC terminal on backbone
tagmCherry
resistance markers :
1277
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA