This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTK21
catalog :
37396
citations: 5
Reference
Wu J, Larreategui Aparicio A, Lambers M, Bodor D, Klaasen S, Tollenaar E, et al. Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression. Curr Biol. 2023;33:912-925.e6 pubmed publisher
Yoo T, Mitchison T. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol. 2021;220: pubmed publisher
Chen M, Nowak D, Narula N, Robinson B, Watrud K, Ambrico A, et al. The nuclear transport receptor Importin-11 is a tumor suppressor that maintains PTEN protein. J Cell Biol. 2017;216:641-656 pubmed publisher
Tame M, Raaijmakers J, Afanasyev P, Medema R. Chromosome misalignments induce spindle-positioning defects. EMBO Rep. 2016;17:317-25 pubmed publisher
Kiyomitsu T, Cheeseman I. Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol. 2012;14:311-7 pubmed publisher
product information
Catalog Number :
37396
Product Name :
pTK21
article :
doi10.1038/ncb2440
id5470
pubmed_id22327364
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Addgene's sequencing result exactly matches the full plasmid sequence provided by the depositing laboratory; however, when these sequences are compared to GenBank ID NP_006316.1 there appears to be a T24N mutation.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SacII
cloning_site_5XhoI
promoter
sequencing_primer_3GCATTCATTTTATGTTTCAGG
sequencing_primer_5GGCATGGACGAGCTGTACAAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARA24, Gsp1, TC4
geneRAN
id5901
genbank_ids
mutationT24N
nameRan
shRNA_sequence
size651
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
locationN terminal on insert
tagTEV
locationN terminal on insert
tagS-tag
resistance markers :
1276
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA