6X-STAT-synthetic luciferase | Addgene 37392 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

6X-STAT-synthetic luciferase

catalog :

37392

citations: 2

Reference
Nataraj N, Noronha A, Lee J, Ghosh S, Mohan Raju H, Sekar A, et al. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors. Cell Rep. 2022;38:110418 pubmed publisher
Baeg G, Zhou R, Perrimon N. Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Genes Dev. 2005;19:1861-70 pubmed

product information

Catalog Number :

37392

Product Name :

6X-STAT-synthetic luciferase

article :

doi	10.1101/gad.1320705
id	5481
pubmed_id	16055650

bacterial resistance :

Ampicillin

cloning :

backbone

pUAST

backbone_mutation

backbone_origin

backbone_size

8904

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

Luciferase

growth notes :

AACGGTTTCCCAGAACGCAGCGTATAAACGGTTTCCGCGAACGCAGCGTATAAACGGTTTCCCAGAATGCA and (2) GCTGCGTTCGCGGAAACCGTTTATACGCTGCGTTCTGGGAAACCGTTTATACGCTGCGTTCGCGGAA, AATTTTCCGCGAACGCAGCGTATAAACGGTTTCCCAGAACGCAGCGTATAAACGGTTTCCGCGAACGCAGCGTATA were annealed, respectively, and cloned together into pUAST. Subsequently, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3 luciferase vector (Promega) and the hsp70 minimal promoter were subcloned into the resulting vector using XhoI/XbaI and NotI/XhoI sites, respectively, to generate the 6XSTAT synthetic-luc construct.
TTCTGGGAAACCGTTTATACGCTGCGTTCGCGGAAACCG
TTTATACGCTGCGTTCTGGGAAACCGTTTATAC,
Perrimon Lab plasmid #445 To generate a reporter construct containing six STAT consensus sites (TCCNNNGGA), two pairs of oligos (1)

origin :

pi :

alt_names

6XSTAT-synthetic-luc

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	Hsp70
sequencing_primer_3
sequencing_primer_5	pUAST-5 (5'-CTGCAACTAAAATCTGCCAAGAAG-3')
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

six STAT consensus sites

shRNA_sequence

size

species

100

Synthetic