pAct5C-Hh | Addgene 37382 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAct5C-Hh

catalog :

37382

citations: 2

Reference
Mafi A, Purohit R, Vielmas E, Lauinger A, Lam B, Cheng Y, et al. Hedgehog proteins create a dynamic cholesterol interface. PLoS ONE. 2021;16:e0246814 pubmed publisher
Nybakken K, Vokes S, Lin T, McMahon A, Perrimon N. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet. 2005;37:1323-32 pubmed

product information

Catalog Number :

37382

Product Name :

pAct5C-Hh

article :

doi	10.1038/ng1682
id	5479
pubmed_id	16311596

bacterial resistance :

Ampicillin

cloning :

backbone

pAct5C

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Perrimon Lab plasmid #440 pAct5C-Hh construct was generated by PCR of full-length hh from a cDNA (gift from T. Kornberg; UCSF) with a 5' BamHI site and a 3' stop followed by a NotI site

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter	actin5C
sequencing_primer_3	SV40pA-R
sequencing_primer_5	AC5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG4637, CG4637, Dmel CG4637, Dmhh, HH, Hg, Hh, Mir, Mrt, anon-WO0134654.19, anon-WO0182946.19, bar, bar-3, bar3, hg, l(3)hh, l(3)neo56, l(3)neo57
gene	hh
id	42737

genbank_ids

mutation

name

hedgehog

shRNA_sequence

size

species

7227

Drosophila melanogaster