Renilla luciferase-Pol III | Addgene 37380 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Renilla luciferase-Pol III

catalog :

37380

citations: 6

Reference
Sayedyahossein S, Hedman A, Sacks D. Insulin suppresses transcriptional activity of yes-associated protein in insulin target cells. Mol Biol Cell. 2020;31:131-141 pubmed publisher
Lindberg B, Tang X, Dantoft W, Gohel P, Seyedoleslami Esfahani S, Lindvall J, et al. Nubbin isoform antagonism governs Drosophila intestinal immune homeostasis. PLoS Pathog. 2018;14:e1006936 pubmed publisher
Qiu Y, Ye X, Zhang H, Hanson P, Zhao G, Tong L, et al. Cleavage of osmosensitive transcriptional factor NFAT5 by Coxsackieviral protease 2A promotes viral replication. PLoS Pathog. 2017;13:e1006744 pubmed publisher
Sayedyahossein S, Li Z, Hedman A, Morgan C, Sacks D. IQGAP1 Binds to Yes-associated Protein (YAP) and Modulates Its Transcriptional Activity. J Biol Chem. 2016;291:19261-73 pubmed publisher
Dantoft W, Davis M, Lindvall J, Tang X, Uvell H, Junell A, et al. The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota. BMC Biol. 2013;11:99 pubmed publisher
Nybakken K, Vokes S, Lin T, McMahon A, Perrimon N. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet. 2005;37:1323-32 pubmed

product information

Catalog Number :

37380

Product Name :

Renilla luciferase-Pol III

article :

doi	10.1038/ng1682
id	5479
pubmed_id	16311596

bacterial resistance :

Ampicillin

cloning :

backbone

pRL-null

backbone_mutation

backbone_origin

Promega

backbone_size

3320

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Perrimon Lab plasmid #414 The PolIII Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224 43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.

origin :

pi :

alt_names

RNA polymerase III control promoter

RNA PolIII 128 subunit (RpIII128)

Renilla luciferase

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	BglII
promoter	RNA PolIII 128 subunit (RpIII128)
sequencing_primer_3	EBV-rev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG8344, CG8344, DmRP128, Dmel CG8344, RP128
gene	RpIII128
id	36289

genbank_ids

AE003823

mutation

name

PolIII-Renilla control reporter

shRNA_sequence

size

species

7227

Drosophila melanogaster