This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMB38-HF380
catalog :
37332
citations: 1
Reference
Kon T, Oyama T, Shimo Kon R, Imamula K, Shima T, Sutoh K, et al. The 2.8?Å crystal structure of the dynein motor domain. Nature. 2012;484:345-50 pubmed publisher
product information
Catalog Number :
37332
Product Name :
pMB38-HF380
article :
doi10.1038/nature10955
id5468
pubmed_id22398446
bacterial resistance :
Ampicillin
cloning :
backbonepMB38
backbone_mutation
backbone_origin
backbone_size8500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Dictyostelium Expression
growth notes :
The backbone plasmid MB38 was created by Mieke Blaauw and Peter van Haastert (Gene 252 (2000) 71 82).
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5BglII
promoterTRE-Pmin
sequencing_primer_3ggaacaatgatcaactcacacc
sequencing_primer_5aatgttaactctgtaatga
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDB_G0276355, DDBDRAFT_0166999, DDBDRAFT_0185096, DDB_0166999, DDB_0185096
genedhcA
id8620457
genbank_ids
mutationdeleted amino acids 1-1387
nameCytoplasmic Dynein Heavy Chain
shRNA_sequence
size10126
species
Dictyostelium discoideum
tags
locationN terminal on insert
tagHis6 and FLAG tag
resistance markers :
1274
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA