pCaShs-Tum | Addgene 37303 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCaShs-Tum

catalog :

37303

citations: 1

Reference
Harrison D, Binari R, Nahreini T, Gilman M, Perrimon N. Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects. EMBO J. 1995;14:2857-65 pubmed

product information

Catalog Number :

37303

Product Name :

pCaShs-Tum

article :

doi
id	5454
pubmed_id	7796812

bacterial resistance :

Ampicillin

cloning :

backbone

pCaShs-hopscotch

backbone_mutation

backbone_origin

Addgene plasmid# 37299

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Perrimon Lab plasmid #101 The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies. This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341.

origin :

pi :

alt_names

Tumorous-lethal

Tum-1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	NotI
promoter	Hsp70
sequencing_primer_3
sequencing_primer_5	pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG1594, 4, CG1594, Dm JAK, DmHD-160, Dmel CG1594, HD-160, HOP, Hop, Hop1, Hop2, Hpo, JAK, Jak, L4, Tum, Tum-1, d-jak, hop/JAK, jak, l(1)10Be, l(1)G18, l(1)L4, l(1)hop, msvl
gene	hop
id	32080

genbank_ids

AAA62441.1

mutation

Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341

name

hopTum-1

shRNA_sequence

size

species

7227

Drosophila melanogaster