This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFoxd3floxtv
catalog :
37268
citations: 1
Reference
Teng L, Mundell N, Frist A, Wang Q, Labosky P. Requirement for Foxd3 in the maintenance of neural crest progenitors. Development. 2008;135:1615-24 pubmed publisher
product information
Catalog Number :
37268
Product Name :
pFoxd3floxtv
article :
doi10.1242/dev.012179
id5437
pubmed_id18367558
bacterial resistance :
Ampicillin
cloning :
backbonepPNT4
backbone_mutationA 1.2 kb fragment of the Foxd3 locus was inserted into the EcoRI site of pPNT4 downstream of the Frt-flanked Neo gene to serve as the 3 homology arm. An oligonucleotide containing the loxP recognition sequence (ATAACTTCGTATAGCATACATTATACGAAGTTAT) was inserted 5 of the Foxd3 coding region. Upstream, a 4.2 kb fragment of the Foxd3 locus was inserted to complete the 5 homology arm to generate the final targeting vector.
backbone_origin
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
Cre/Lox
origin :
37
pi :
alt_names
Hfh2
cloning
entrez_gene
aliasesCWH3, Gen, Genesis, Hfh2
geneFoxd3
id15221
genbank_ids
1347473
mutation
nameFoxd3
shRNA_sequence
size16000
species
10090
Mus musculus
tags
resistance markers :
1251
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA