This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFoxd3IRESGFPtv
catalog :
37266
citations: 1
| Reference |
|---|
Hanna L, Foreman R, Tarasenko I, Kessler D, Labosky P. Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo. Genes Dev. 2002;16:2650-61 pubmed
|
product information
Catalog Number :
37266
Product Name :
pFoxd3IRESGFPtv
article :
| doi | 10.1101/gad.1020502 |
| id | 5435 |
| pubmed_id | 12381664 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBS | |
| backbone_mutation | The targeting vector was constructed using a 4.0 kilobase (kb) Sac1 fragment as the 5homology arm and a 1.5kb Xba1 fragment as the 3arm. A diptheria toxin (DTA) cassette was used to select against random integration, and positive selection was provided by the neo cassette, flanked by loxP sites for subsequent removal in culture. The Foxd3 targeted allele has the entire coding region deleted as well as 70 bp of the 3 flanking DNA. A Gfp gene with an internal ribosomal entry site (Ires, for the Foxd3Ires-Gfp allele) was inserted as described in Hanna et al. | |
| backbone_origin | Stratgene | |
| backbone_size | 3000 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
origin :
37
pi :
|
resistance markers :
| 1251 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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