This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMf3tvtaulacZ
catalog :
37264
citations: 1
| Reference |
|---|
Kloetzli J, Fontaine Glover I, Brown E, Kuo M, Labosky P. The winged helix gene, Foxb1, controls development of mammary glands and regions of the CNS that regulate the milk-ejection reflex. Genesis. 2001;29:60-71 pubmed
|
product information
Catalog Number :
37264
Product Name :
pMf3tvtaulacZ
article :
| doi | 10.1002/1526-968X(200102)29:2<60::AID-GENE1006>3.0.CO;2-L |
| id | 5434 |
| pubmed_id | 11170346 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pPNT |
| backbone_mutation | The targeting vector was constructed using 129Sv/J DNA. A 3.5kb EcoR1 fragment was used as the 39 homology arm and subcloned into a modified version of pPNT with a Not1 site introduced into the Sal1 site for linearization. An 8.5 kb BamH1/BglII fragment was used as the 59 homology arm. A tau-lacZ-PGKneo cassette (generous gift of Peter Mombaerts) was inserted between these two arms to give the vector described in Kloetzli et al. This deletion construct results in the replacement of the entire coding region of Foxb1 with the tau-lacZ fusion gene and the PGKneo cassette. The resulting mutation is a null. |
| backbone_origin | |
| backbone_size | 3000 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types |
origin :
37
pi :
|
resistance markers :
| 1251 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments