This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC19-DAD4
catalog :
37224
citations: 1
Reference
Mitchell S, Walker S, Algire M, Park E, Hinnebusch A, Lorsch J. The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway. Mol Cell. 2010;39:950-62 pubmed publisher
product information
Catalog Number :
37224
Product Name :
pUC19-DAD4
article :
doi10.1016/j.molcel.2010.08.021
id5420
pubmed_id20864040
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size2686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The sequence for DAD4 mRNA was taken from yeast.utgenome.org. Two G bases were added at the 5 -ends of the mRNA in order to promote efficient transcription and the sequence GGATC was added at the 3 -end as part of a BamHI site during cloning.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3M13-pUC-Rev
sequencing_primer_5M13pUC-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR320C-A, HSK2
geneDAD4
id851919
genbank_ids
mutation
nameDAD4
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
1269
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA