This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEYFP-N1-A2BR
catalog :
37202
citations: 5
Reference
Nishiyama K, Nishimura A, Shimoda K, Tanaka T, Kato Y, Shibata T, et al. Redox-dependent internalization of the purinergic P2Y6 receptor limits colitis progression. Sci Signal. 2022;15:eabj0644 pubmed publisher
Alkhatib O, da Costa R, Gentry C, Quallo T, Bevan S, Andersson D. Promiscuous G-Protein-Coupled Receptor Inhibition of Transient Receptor Potential Melastatin 3 Ion Channels by Gβγ Subunits. J Neurosci. 2019;39:7840-7852 pubmed publisher
Burda P, Caldelari R, Heussler V. Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress. MBio. 2017;8: pubmed publisher
Jones K, Choi U, Gao J, THOMPSON R, Rodman L, Malech H, et al. A Novel Method for Screening Adenosine Receptor Specific Agonists for Use in Adenosine Drug Development. Sci Rep. 2017;7:44816 pubmed publisher
Watson M, Worthington E, Clunes L, Rasmussen J, Jones L, Tarran R. Defective adenosine-stimulated cAMP production in cystic fibrosis airway epithelia: a novel role for CFTR in cell signaling. FASEB J. 2011;25:2996-3003 pubmed publisher
product information
Catalog Number :
37202
Product Name :
pEYFP-N1-A2BR
article :
doi10.1096/fj.11-186080
id5414
pubmed_id21628448
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-N1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A2B was PCR amplified as a 1068bp fragment incorporating a 5' EcoRI site. The stop codon was eliminated and a 3' BamHI site was added with the reverse PCR primer to allow direct cloning into pEYFP-N1
origin :
37
pi :
alt_names
adenosine A2b receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADORA2
geneADORA2B
id136
genbank_ids
mutation
nameA2BR
shRNA_sequence
size1068
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEYFP
resistance markers :
1266
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA