This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-SypHluorin 4x (S4x)
catalog :
37005
citations: 5
Reference
Kim D, Park S, Ye M, Chen J, Jhang J, Hunker A, et al. Novel genetically encoded tools for imaging or silencing neuropeptide release from presynaptic terminals in vivo. bioRxiv. 2023;: pubmed publisher
Lam M, Takeo K, Almeida R, Cooper M, Wu K, Iyer M, et al. CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes. Nat Commun. 2022;13:5583 pubmed publisher
Almeida R, Williamson J, Madden M, Early J, Voas M, Talbot W, et al. Myelination induces axonal hotspots of synaptic vesicle fusion that promote sheath growth. Curr Biol. 2021;: pubmed publisher
Hammond J, Lu S, Gelbard H. Platelet Activating Factor Enhances Synaptic Vesicle Exocytosis Via PKC, Elevated Intracellular Calcium, and Modulation of Synapsin 1 Dynamics and Phosphorylation. Front Cell Neurosci. 2015;9:505 pubmed publisher
Zhu Y, Xu J, Heinemann S. Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009;61:397-411 pubmed publisher
product information
Catalog Number :
37005
Product Name :
pcDNA3-SypHluorin 4x (S4x)
article :
doi10.1016/j.neuron.2008.12.024
id5346
pubmed_id19217377
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSyp1
geneSyp
id24804
genbank_ids
mutationFour copies of pHluorin inserted at site 184-185
nameSypHluorin 4x
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagHA
resistance markers :
1242
1245
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA