This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-SypHluorin 2x (S2x)
catalog :
37004
citations: 6
Reference
Speigel I, Patel K, Osman V, Hemmings H. Volatile anesthetics inhibit presynaptic cGMP signaling to depress presynaptic excitability in rat hippocampal neurons. Neuropharmacology. 2023;240:109705 pubmed publisher
Osman V, Speigel I, Patel K, Hemmings H. Isoflurane Alters Presynaptic Endoplasmic Reticulum Calcium Dynamics in Wild-Type and Malignant Hyperthermia-Susceptible Rodent Hippocampal Neurons. Eneuro. 2023;10: pubmed publisher
Kim S, Eom Y, Lee S. Comprehensive analysis of sex differences in the function and ultrastructure of hippocampal presynaptic terminals. Neurochem Int. 2023;169:105570 pubmed publisher
Shi B, Wu X, Cordero N, Moreira S, Wu L. Light and electron microscopic imaging of synaptic vesicle endocytosis at mouse hippocampal cultures. STAR Protoc. 2022;3:101495 pubmed publisher
Dimitrov D, Takagi H, Guillaud L, Saitoh N, Eguchi K, Takahashi T. Reconstitution of Giant Mammalian Synapses in Culture for Molecular Functional and Imaging Studies. J Neurosci. 2016;36:3600-10 pubmed publisher
Zhu Y, Xu J, Heinemann S. Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009;61:397-411 pubmed publisher
product information
Catalog Number :
37004
Product Name :
pcDNA3-SypHluorin 2x (S2x)
article :
doi10.1016/j.neuron.2008.12.024
id5346
pubmed_id19217377
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSyp1
geneSyp
id24804
genbank_ids
mutationTwo copies of pHluorin inserted at site 184-185
nameSypHluorin 2x
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagHA
resistance markers :
1242
1245
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA