pLV-beta-catenin deltaN90 | Addgene 36985 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLV-beta-catenin deltaN90

catalog :

36985

citations: 5

Reference
Strash N, DeLuca S, Janer Carattini G, Heo S, Gorsuch R, Bursac N. Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocytes. elife. 2021;10: pubmed publisher
Abreu de Oliveira W, Moens S, El Laithy Y, van der Veer B, Athanasouli P, Cortesi E, et al. Wnt/β-Catenin Inhibition Disrupts Carboplatin Resistance in Isogenic Models of Triple-Negative Breast Cancer. Front Oncol. 2021;11:705384 pubmed publisher
Li Q, Sun Y, Jarugumilli G, Liu S, Dang K, Cotton J, et al. Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. Cell Stem Cell. 2020;26:675-692.e8 pubmed publisher
Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. PLoS ONE. 2019;14:e0226570 pubmed publisher
Guo W, Keckesova Z, Donaher J, Shibue T, Tischler V, Reinhardt F, et al. Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012;148:1015-28 pubmed publisher

product information

Catalog Number :

36985

Product Name :

pLV-beta-catenin deltaN90

article :

doi	10.1016/j.cell.2012.02.008
id	5338
pubmed_id	22385965

bacterial resistance :

Ampicillin

cloning :

backbone

pLV-IRES-neo

backbone_mutation

pLV-IRES-neo (a gift from I. Ben-Porath) was generated by replacing dsRed2 in pLV-tTRKRAB-Red (Addgene plasmid 12250) with the neomycin-resistance gene and removing the sequence of tTR-KRAB.

backbone_origin

I. Ben-Porath

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB. Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.

origin :

pi :

alt_names

beta-catenin

CTNNB1

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3	WPRE-R
sequencing_primer_5	EF1a-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CTNNB, EVR7, MRD19, NEDSDV, armadillo
gene	CTNNB1
id	1499

genbank_ids

mutation

Deletion of amino acids 1-90 (Constitutively Active); S374G in beta-catenin

name

beta-catenin deltaN90

shRNA_sequence

size

species

9606

Homo sapiens