FLAG-EglN3-pLenti6 | Addgene 36951 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

FLAG-EglN3-pLenti6

catalog :

36951

citations: 3

Reference
Cai X, Yuan Y, Liao Z, Xing K, Zhu C, Xu Y, et al. α-Ketoglutarate prevents skeletal muscle protein degradation and muscle atrophy through PHD3/ADRB2 pathway. FASEB J. 2018;32:488-499 pubmed publisher
Lee G, Won H, Lee Y, Choi J, Oh T, Jang J, et al. Oxidative Dimerization of PHD2 is Responsible for its Inactivation and Contributes to Metabolic Reprogramming via HIF-1Î± Activation. Sci Rep. 2016;6:18928 pubmed publisher
Koivunen P, Lee S, Duncan C, Lopez G, Lu G, Ramkissoon S, et al. Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to EGLN activation. Nature. 2012;483:484-8 pubmed publisher

product information

Catalog Number :

36951

Product Name :

FLAG-EglN3-pLenti6

article :

doi	10.1038/nature10898
id	5273
pubmed_id	22343896

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti6

backbone_mutation

the plenti6-V5 GW LacZ backbone of Invitrogen was modified to delete the original lacz ORF as well as attR sites and V5-tag and replaced with the Flag-ORF

backbone_origin

Invitrogen

backbone_size

6887

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

this plasmid was constructed in the lab of a HHMI-investigator

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoR I
cloning_site_5	Xba I
promoter	CMV
sequencing_primer_3	ACACCTGGTTGCTGACTAAT
sequencing_primer_5	CMV-forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HIFP4H3, HIFPH3, PHD3
gene	EGLN3
id	112399

genbank_ids

mutation

Wildtype

name

EglN3

shRNA_sequence

size

species

9606

Homo sapiens