This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3/Myc-DNMT3A2
catalog :
36941
citations: 3
Reference
Bayraktar G, Yuanxiang P, Confettura A, Gomes G, Raza S, Stork O, et al. Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus. Neuropsychopharmacology. 2020;45:2120-2130 pubmed publisher
Finnegan A, Kim S, Jin H, Gapinske M, Woods W, Perez Pinera P, et al. Epigenetic engineering of yeast reveals dynamic molecular adaptation to methylation stress and genetic modulators of specific DNMT3 family members. Nucleic Acids Res. 2020;48:4081-4099 pubmed publisher
Chen Z, Mann J, Hsieh C, Riggs A, Chedin F. Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family. J Cell Biochem. 2005;95:902-17 pubmed
product information
Catalog Number :
36941
Product Name :
pcDNA3/Myc-DNMT3A2
article :
doi10.1002/jcb.20447
id5141
pubmed_id15861382
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT3A2 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3see comments
cloning_site_5see comments
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
AF480163
mutation
nameDNMT3A2
shRNA_sequence
size2070
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyc
resistance markers :
1181
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA