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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3/Myc-DNMT1
catalog :
36939
citations: 26
Reference
Shin H, Leung A, Costello K, Senapati P, Kato H, Moore R, et al. Inhibition of DNMT1 methyltransferase activity via glucose-regulated O-GlcNAcylation alters the epigenome. elife. 2023;12: pubmed publisher
Okuda K, Nakahara K, Ito A, Iijima Y, Nomura R, Kumar A, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. Nat Commun. 2023;14:621 pubmed publisher
Liu H, Liu Y, Wang H, Zhao Q, Zhang T, Xie S, et al. Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation. Adv Sci (Weinh). 2022;9:e2203995 pubmed publisher
Switzer C, Cho H, Eykyn T, Lavender P, Eaton P. NOS2 and S-nitrosothiol signaling induces DNA hypomethylation and LINE-1 retrotransposon expression. Proc Natl Acad Sci U S A. 2022;119:e2200022119 pubmed publisher
Sharma V, Joshi J, Yeh I, Doughman Y, Blankenberg D, Wald D, et al. Re-Expression of ERα and AR in Receptor Negative Endocrine Cancers via GSK3 Inhibition. Front Oncol. 2022;12:824594 pubmed publisher
Wang J, Xie S, Li N, Zhang T, Yao W, Zhao H, et al. Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. Bioact Mater. 2022;17:406-424 pubmed publisher
Huang X, Zhang X, Zong L, Gao Q, Zhang C, Wei R, et al. Gene body methylation safeguards ribosomal DNA transcription by preventing PHF6-mediated enrichment of repressive histone mark H4K20me3. J Biol Chem. 2021;297:101195 pubmed publisher
Kaplun D, Starshin A, Sharko F, Gainova K, Filonova G, Zhigalova N, et al. Kaiso Regulates DNA Methylation Homeostasis. Int J Mol Sci. 2021;22: pubmed publisher
Zhang X, Zhao S, Yuan Q, Zhu L, Li F, Wang H, et al. TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression. Cell Death Dis. 2021;12:642 pubmed publisher
Cao K, Lv W, Wang X, Dong S, Liu X, Yang T, et al. Hypermethylation of Hepatic Mitochondrial ND6 Provokes Systemic Insulin Resistance. Adv Sci (Weinh). 2021;8:2004507 pubmed publisher
Finnegan A, Kim S, Jin H, Gapinske M, Woods W, Perez Pinera P, et al. Epigenetic engineering of yeast reveals dynamic molecular adaptation to methylation stress and genetic modulators of specific DNMT3 family members. Nucleic Acids Res. 2020;48:4081-4099 pubmed publisher
LIU Y, Zhu J, Yu Tian X, Liu H, Zhang T, Zhang Y, et al. Hypermethylation of mitochondrial DNA in vascular smooth muscle cells impairs cell contractility. Cell Death Dis. 2020;11:35 pubmed publisher
Liu J, Banerjee A, Herring C, Attalla J, Hu R, Xu Y, et al. Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor Identity. Dev Cell. 2019;48:49-63.e7 pubmed publisher
Lyu G, Zong L, Zhang C, Huang X, Xie W, Fang J, et al. Metastasis-related methyltransferase 1 (Merm1) represses the methyltransferase activity of Dnmt3a and facilitates RNA polymerase I transcriptional elongation. J Mol Cell Biol. 2018;: pubmed publisher
Huang M, Chou Y, Li M, Shih T, Lin S, Chuang H, et al. Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition. Pharmacol Res. 2019;139:50-61 pubmed publisher
Lima F, Seba V, Silva G, Torrezan G, Polaquini C, Pinhanelli V, et al. The Curcumin Analog CH-5 Exerts Anticancer Effects in Human Osteosarcoma Cells via Modulation of Transcription Factors p53/Sp1. Int J Mol Sci. 2018;19: pubmed publisher
Lin L, Liu Y, Xu F, Huang J, Daugaard T, Petersen T, et al. Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. Gigascience. 2018;7:1-19 pubmed publisher
Shi H, Chen X, Jiang H, Wang X, Yu H, Sun P, et al. miR-148a suppresses cell invasion and migration in gastric cancer by targeting DNA methyltransferase 1. Oncol Lett. 2018;15:4944-4950 pubmed publisher
Tan X, Banerjee P, Liu X, Yu J, Gibbons D, Wu P, et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest. 2018;128:1267-1282 pubmed publisher
Xie S, Zhang T, Wang J, Zhao F, Zhang Y, Yao W, et al. Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo: Role of DNA methyltransferase 1. Biomaterials. 2018;155:203-216 pubmed publisher
Peng X, Chang H, Chen J, Zhang Q, Yu X, Mi M. 3,6-Dihydroxyflavone regulates microRNA-34a through DNA methylation. BMC Cancer. 2017;17:619 pubmed publisher
Goel S, Decristo M, Watt A, BrinJones H, Sceneay J, Li B, et al. CDK4/6 inhibition triggers anti-tumour immunity. Nature. 2017;548:471-475 pubmed publisher
Schnepp P, Lee D, Guldner I, O Tighearnaigh T, Howe E, Palakurthi B, et al. GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment. Cancer Res. 2017;77:2844-2856 pubmed publisher
Garella D, Atlante S, Borretto E, Cocco M, Giorgis M, Costale A, et al. Design and synthesis of N-benzoyl amino acid derivatives as DNA methylation inhibitors. Chem Biol Drug Des. 2016;88:664-676 pubmed publisher
Fatima S, Wagstaff K, Loveland K, Jans D. Interactome of the negative regulator of nuclear import BRCA1-binding protein 2. Sci Rep. 2015;5:9459 pubmed publisher
Li H, Rauch T, Chen Z, SZABO P, Riggs A, Pfeifer G. The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells. J Biol Chem. 2006;281:19489-500 pubmed
product information
Catalog Number :
36939
Product Name :
pcDNA3/Myc-DNMT1
article :
doi10.1074/jbc.M513249200
id5333
pubmed_id16682412
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationpcDNA3/Myc was constructed by Zhaoxia Chen (see Comments section for details). DNMT1 was cloned into the pcDNA3/Myc vector.
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT1 was cloned into EcoRI and NotI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
growth strain :
Mammalian expression of human DNMT1 with myc tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI
geneDNMT1
id1786
genbank_ids
X63692
mutation
nameDNMT1
shRNA_sequence
size4851
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyc
resistance markers :
1181
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA