This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TAL2456
catalog :
36845
citations: 1
Reference
Reyon D, Tsai S, Khayter C, Foden J, Sander J, Joung J. FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol. 2012;30:460-5 pubmed publisher
product information
Catalog Number :
36845
Product Name :
TAL2456
article :
doi10.1038/nbt.2170
id5318
pubmed_id22484455
bacterial resistance :
Ampicillin
cloning :
backboneJDS70
backbone_mutation
backbone_originJoung lab (Addgene Plasmid # 32285)
backbone_size6447
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Mammalian, T7 for in vitro mRNA synthesis
growth notes :
Target binding site: TCTGGATCGCGGAGGGAA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
origin :
37
pi :
alt_names
VHL
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesVhlh, pVHL
geneVhl
id22346
genbank_ids
mutation
nameVHL-TALEN_Left
shRNA_sequence
size1640
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3X Flag
locationC terminal on backbone
tagWT FOKI
resistance markers :
236
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA