This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TAL2255
catalog :
36698
citations: 2
Reference
Okamoto Y, Iwasaki W, Kugou K, Takahashi K, Oda A, Sato K, et al. Replication stress induces accumulation of FANCD2 at central region of large fragile genes. Nucleic Acids Res. 2018;46:2932-2944 pubmed publisher
Reyon D, Tsai S, Khayter C, Foden J, Sander J, Joung J. FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol. 2012;30:460-5 pubmed publisher
product information
Catalog Number :
36698
Product Name :
TAL2255
article :
doi10.1038/nbt.2170
id5318
pubmed_id22484455
bacterial resistance :
Ampicillin
cloning :
backboneJDS71
backbone_mutation
backbone_originJoung lab (Addgene Plasmid # 32287)
backbone_size6447
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Mammalian, T7 for in vitro mRNA synthesis
growth notes :
Target binding site: TCTACTGAATCTTGAGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
origin :
37
pi :
alt_names
FANCC
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBB116513, Facc
geneFancc
id14088
genbank_ids
mutation
nameFANCC-TALEN_Right
shRNA_sequence
size1538
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3X Flag
locationC terminal on backbone
tagWT FOKI
resistance markers :
236
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA