Bcr del oligo pCDNA3.1/HisB | Addgene 36423 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr del oligo pCDNA3.1/HisB

catalog :

36423

citations: 1

Reference
Yi S, Groffen J, Heisterkamp N. Bcr is a substrate for Transglutaminase 2 cross-linking activity. BMC Biochem. 2011;12:8 pubmed publisher

product information

Catalog Number :

36423

Product Name :

Bcr del oligo pCDNA3.1/HisB

article :

doi	10.1186/1471-2091-12-8
id	5300
pubmed_id	21310073

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA3.1/hisB

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

To generate Xpress-tagged Bcr del oligo, we isolated the N-terminal end of Bcr with deletion of amino acid residues 2-63 from a previously described 474 bp subclone by digestion with Eag I x Xho I.The 3.5 kb 3 end of BCR was isolated from a cDNA clone in pSK (B1/SK) by digestion with Xho I x Xba I. These two fragments were combined in pcDNA3.1/HisB digested with Not I x Xba. Construct has a deletion of amino acid residues 2-63. The first amino acid residues are M- AAA- KEKKSY. The AAA originate from the pSL1180 polylinker, The KEKK. are Bcr amino acids. Constructed by Sun-ju Yi.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xba I
cloning_site_5	EagI
promoter
sequencing_primer_3	BGH-Rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

Deletion of amino acid residues 2-63

name

BCR

shRNA_sequence

size

species

9606

Homo sapiens