Bcr del PK | Addgene 36422 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr del PK

catalog :

36422

citations: 1

Reference
Yi S, Groffen J, Heisterkamp N. Transglutaminase 2 regulates the GTPase-activating activity of Bcr. J Biol Chem. 2009;284:35645-51 pubmed publisher

product information

Catalog Number :

36422

Product Name :

Bcr del PK

article :

doi	10.1074/jbc.M109.062240
id	5299
pubmed_id	19840940

bacterial resistance :

Ampicillin

cloning :

backbone

pCDE

backbone_mutation

Polylinker from mp13 inserted into the sole cloning site -EcoRI- in the original vector pCD to make pCDE.

backbone_origin

p CD from Paul Berg

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Construct includes 5' untranslated sequences, and the first 39 aminoacids including a small part of the N-terminal oligomerization domain. The deletion was generated by ligation of a Stu I site in BCR exon 1 to a downstream Nae I site. The construct lacks the ST kinase domain. Vector is a derivative of pCD with a multiple cloning site included. Has SV-40 origin of replication for high yield expression in COS-1 cells. Also see Yi et al 2009. Constructed by Vesa Kaartinen.

origin :

pi :

alt_names

cloning

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

deletion of residues 40-447

name

BCR

shRNA_sequence

size

species

9606

Homo sapiens