ECFP-Bcr NE/AA | Addgene 36418 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ECFP-Bcr NE/AA

catalog :

36418

citations: 2

Reference
Duman J, Mulherkar S, Tu Y, Erikson K, Tzeng C, Mavratsas V, et al. The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest. elife. 2019;8: pubmed publisher
Cho Y, Cunnick J, Yi S, Kaartinen V, Groffen J, Heisterkamp N. Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Mol Cell Biol. 2007;27:899-911 pubmed

product information

Catalog Number :

36418

Product Name :

ECFP-Bcr NE/AA

article :

doi	10.1128/MCB.00756-06
id	4686
pubmed_id	17116687

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.

origin :

pi :

alt_names

BCR

cloning

clone_method	Restriction Enzyme
cloning_site_3	Kpn I
cloning_site_5	BamHI
promoter
sequencing_primer_3	SV40pA-R
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

N689A/ E690A

name

breakpoint cluster region

shRNA_sequence

size

4300

species

9606

Homo sapiens