Bcr GAP domain R1090A/pGEX 3X | Addgene 36406 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr GAP domain R1090A/pGEX 3X

catalog :

36406

citations: 1

Reference
Cho Y, Cunnick J, Yi S, Kaartinen V, Groffen J, Heisterkamp N. Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Mol Cell Biol. 2007;27:899-911 pubmed

product information

Catalog Number :

36406

Product Name :

Bcr GAP domain R1090A/pGEX 3X

article :

doi	10.1128/MCB.00756-06
id	4686
pubmed_id	17116687

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX 3X

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

We constructed this in a 3-way ligation. The 5' end was a 0.16 kb BamHI-BstEII fragment from plasmid Bcr GAP domain in pGEX-3X, which provided the 5' BamHI site from pGEX-3X. The 3' end was a 1.2 kb BstEII-EcoRI fragment carrying the R1090A mutation. This was cloned into pGEX-3X digested with BamHI-EcoRI. The construct encodes the GAP-dead domain of Bcr which is inactive against GTP-bound Rac. It includes the natural stop codon and 3' untranslated sequences of the cDNA. Constructed by Nora Heisterkamp. The Rosetta2 strain may improve yield of the fusion protein.

inserts :

Rosetta2

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pGEX-3X
sequencing_primer_5	pGEX-3X
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

name

Bcr GAP domain encoding residues 1004-1271 with R1090A point mutation

shRNA_sequence

size

1300

species

9606

Homo sapiens