This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-2T Crkl SH3SH3
catalog :
36403
citations: 1
Reference
De Jong R, ten Hoeve J, Heisterkamp N, Groffen J. Crkl is complexed with tyrosine-phosphorylated Cbl in Ph-positive leukemia. J Biol Chem. 1995;270:21468-71 pubmed
product information
Catalog Number :
36403
Product Name :
pGEX-2T Crkl SH3SH3
article :
doi10.1074/jbc.270.37.21468
id5295
pubmed_id7545163
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2T
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This construct includes the N- and C-terminal SH3 domains of Crkl, including residues 115-303. It is a 630 bp BsmI fragment (blunt) cloned into the SmaI site of pGEX-2T. Constructed by johanna ten Hoeve. Additional reference PMID 9053848.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sma I
cloning_site_5Sma I
promoter
sequencing_primer_3pGEX-3
sequencing_primer_5pGEX-5
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases
geneCRKL
id1399
genbank_ids
mutation
nameCrkl tandem SH3 domains
shRNA_sequence
size630
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST
resistance markers :
995
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA