This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRL WS1.6 WASp
catalog :
36250
citations: 1
Reference
Astrakhan A, Sather B, Ryu B, Khim S, Singh S, Humblet Baron S, et al. Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich syndrome. Blood. 2012;119:4395-407 pubmed publisher
product information
Catalog Number :
36250
Product Name :
pRRL WS1.6 WASp
article :
doi10.1182/blood-2011-03-340711
id5271
pubmed_id22431569
bacterial resistance :
Ampicillin
cloning :
backbonepRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252
backbone_mutationPGK GFP removed (EcoRV and BamHI)and replaced with a multiple cloning site. WS1.6-WASp cassette cloned into MluI-SpeI site of new MCS.
backbone_originDidier Trono
backbone_size6857
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
origin :
37
pi :
alt_names
Wiskott-Aldrich Syndrome Protein
WAS
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5MluI
promoterWS1.6
sequencing_primer_3caacgggccacaactcctc
sequencing_primer_5gatcacgagactagcctcgag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIMD2, SCNX, THC, THC1, WASP, WASPA
geneWAS
id7454
genbank_ids
mutation
nameWASp
shRNA_sequence
size1509
species
9606
Homo sapiens
tags
plasmid copy :
Backbone is from Addgene plasmid 12252 (Didier Trono)
resistance markers :
1223
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA