pCMV-calpainsensor | Addgene 36182 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-calpainsensor

catalog :

36182

citations: 3

Reference
Lostal W, Stockholm D, Richard I. In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer. Methods Mol Biol. 2019;1915:57-66 pubmed publisher
Jacquemet G, Baghirov H, Georgiadou M, Sihto H, Peuhu E, Cettour Janet P, et al. L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling. Nat Commun. 2016;7:13297 pubmed publisher
Stockholm D, Bartoli M, Sillon G, Bourg N, Davoust J, Richard I. Imaging calpain protease activity by multiphoton FRET in living mice. J Mol Biol. 2005;346:215-22 pubmed

product information

Catalog Number :

36182

Product Name :

pCMV-calpainsensor

article :

doi	10.1016/j.jmb.2004.11.039
id	5259
pubmed_id	15663939

bacterial resistance :

Kanamycin

cloning :

and 5 -P-GATCCCCTGAGCCATCCCAGGGCATAGCACCATACACCTCCTGCTGGCCGCTT-3 , which encode the linkers and the mouse -fodrin cleavage site by ubiquitous calpains. The peptide sequence of the linker and the -fodrin cleavage site is GSG-QQEVY GAMPRDGSG. GSG corresponds to linker sequencesbackbone_originClontechbackbone_sizepromotersequencing_primer_3sequencing_primer_5vector_types

Mammalian Expression

-PCCGGAAGCGGCCAGCAGGAGGTGTATGGTGCGATGCC
CAGGGATGGCTCAGGG-3

backbone

peYFP

backbone_mutation

Plasmids peYFP and peCFP were obtained from Clontech. pTOM plasmid, containing eYFP and eCFP, respectively, in 5 and 3 of the multi-cloning site was obtained after digestion of peYFP and peCFP by XhoI and HpaI and religation of the eCFP fragment in peYFP. pCalpain-sensor was obtained by digestion of pTOM with BspeI and BamHI and ligation of the following primers: 5

growth strain :

detection of activity of ubiquitous calpains

origin :

pi :

alt_names

calpain sensor

EYFP--calpain clevage site from alpha-Fodrin--ECFP

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamH1
cloning_site_5	Bspe1
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2610027H02Rik, Spna-2, Spna2
gene	Sptan1
id	20740

genbank_ids

mutation

name

alpha-Fodrin cleavage site

shRNA_sequence

size

species

10090

Mus musculus