This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CD200CD4d3+4-His
catalog :
36152
citations: 2
Reference
Gonzales S, Clarke K, Batugedara G, Garza R, Braddom A, Reyes R, et al. A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From Uganda. Front Immunol. 2022;13:809264 pubmed publisher
Bushell K, Söllner C, Schuster Boeckler B, Bateman A, Wright G. Large-scale screening for novel low-affinity extracellular protein interactions. Genome Res. 2008;18:622-30 pubmed publisher
product information
Catalog Number :
36152
Product Name :
CD200CD4d3+4-His
article :
doi10.1101/gr.7187808
id4652
pubmed_id18296487
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutation
backbone_originYves Durocher, NRC
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid contains an intentional G2A mutation in the CD200 sequence to create a consensus Kozak sequence
growth strain :
A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and prey (rat CD200) EXPRESs plasmid
origin :
37
pi :
alt_names
OX-2
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCspmo2, MRCOX2, Mox2
geneCd200
id24560
genbank_ids
mutationG2A
nameCD200
shRNA_sequence
size714
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagratCD4d3+4
locationC terminal on backbone
tagHis tag
plasmid copy :
pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
resistance markers :
919
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA