This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
unc5bCD4d3+4-blac
catalog :
36149
citations: 2
Reference
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens H, Svergun D, et al. Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron. 2018;97:1261-1267.e4 pubmed publisher
Sollner C, Wright G. A cell surface interaction network of neural leucine-rich repeat receptors. Genome Biol. 2009;10:R99 pubmed publisher
product information
Catalog Number :
36149
Product Name :
unc5bCD4d3+4-blac
article :
doi10.1186/gb-2009-10-9-r99
id5250
pubmed_id19765300
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutation
backbone_originYves Durocher, NRC
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.
growth strain :
EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-blac tag
origin :
37
pi :
alt_names
unc5b
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Foward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesunc5h2
geneunc5b
id494130
genbank_ids
mutationL2V
nameunc5b
shRNA_sequence
size1150
species
7955
Danio rerio
tags
locationC terminal on backbone
tagratCD4d3+4
locationC terminal on backbone
tagpentamerization domain of rat cartilaginous oligomeric matrix protein (COMP)
locationC terminal on backbone
tagbeta-lactamase
plasmid copy :
pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
resistance markers :
919
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA