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product summary
company name :
Addgene
product type :
cDNA
product name :
pT7-7 asyn WT
catalog :
36046
citations: 22
Reference
Br xe4 uer S, Rossi M, Sajapin J, Henle T, Gasser T, Parchi P, et al. Kinetic parameters of alpha-synuclein seed amplification assay correlate with cognitive impairment in patients with Lewy body disorders. Acta Neuropathol Commun. 2023;11:162 pubmed publisher
Tahirbegi B, Magness A, Piersimoni M, Teng X, Hooper J, Guo Y, et al. Toward high-throughput oligomer detection and classification for early-stage aggregation of amyloidogenic protein. Front Chem. 2022;10:967882 pubmed publisher
Pieger K, Schmitt V, Gauer C, Gie xdf l N, Prots I, Winner B, et al. Translocation of Distinct Alpha Synuclein Species from the Nucleus to Neuronal Processes during Neuronal Differentiation. Biomolecules. 2022;12: pubmed publisher
Kluge A, Bunk J, Schaeffer E, Drobny A, Xiang W, Knacke H, et al. Detection of neuron-derived pathological α-synuclein in blood. Brain. 2022;: pubmed publisher
Sanders H, Kostelic M, Zak C, Marty M. Lipids and EGCG Affect α-Synuclein Association and Disruption of Nanodiscs. Biochemistry. 2022;61:1014-1021 pubmed publisher
Lau D, Magnan C, Hill K, COOPER A, Gambin Y, Sierecki E. Single Molecule Fingerprinting Reveals Different Amplification Properties of α-Synuclein Oligomers and Preformed Fibrils in Seeding Assay. ACS Chem Neurosci. 2022;13:883-896 pubmed publisher
Ardah M, Eid N, Kitada T, Haque M. Ellagic Acid Prevents α-Synuclein Aggregation and Protects SH-SY5Y Cells from Aggregated α-Synuclein-Induced Toxicity via Suppression of Apoptosis and Activation of Autophagy. Int J Mol Sci. 2021;22: pubmed publisher
Jos S, Gogoi H, Prasad T, Hurakadli M, Kamariah N, Padmanabhan B, et al. Molecular insights into α-synuclein interaction with individual human core histones, linker histone, and dsDNA. Protein Sci. 2021;30:2121-2131 pubmed publisher
Kumar S, Kumar R, Kumari M, Kumari R, Saha S, Bhavesh N, et al. Ellagic Acid Inhibits α-Synuclein Aggregation at Multiple Stages and Reduces Its Cytotoxicity. ACS Chem Neurosci. 2021;12:1919-1930 pubmed publisher
Trinkaus V, Riera Tur I, Martinez Sanchez A, Bäuerlein F, Guo Q, Arzberger T, et al. In situ architecture of neuronal α-Synuclein inclusions. Nat Commun. 2021;12:2110 pubmed publisher
Sharma R, Srivastava T, Pandey A, Mishra T, Gupta B, Reddy S, et al. Identification of Natural Products as Potential Pharmacological Chaperones for Protein Misfolding Diseases. ChemMedChem. 2021;16:2146-2156 pubmed publisher
Luchinat E, Barbieri L, Cremonini M, Banci L. Protein in-cell NMR spectroscopy at 1.2 GHz. J Biomol NMR. 2021;75:97-107 pubmed publisher
Merghani M, Ardah M, Al Shamsi M, Kitada T, Haque M. Dose-related biphasic effect of the Parkinson's disease neurotoxin MPTP, on the spread, accumulation, and toxicity of α-synuclein. Neurotoxicology. 2021;: pubmed publisher
Hou S, Exell J, Welsher K. Real-time 3D single molecule tracking. Nat Commun. 2020;11:3607 pubmed publisher
Lin X, Galaqin N, Tainaka R, Shimamori K, Kuragano M, Noguchi T, et al. Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots. Int J Mol Sci. 2020;21: pubmed publisher
Kumar R, Kumar S, Hanpude P, Singh A, Johari T, Majumder S, et al. Partially oxidized DJ-1 inhibits α-synuclein nucleation and remodels mature α-synuclein fibrils in vitro. Commun Biol. 2019;2:395 pubmed publisher
Shirasaka M, Kuwata K, Honda R. α-Synuclein chaperone suppresses nucleation and amyloidogenesis of prion protein. Biochem Biophys Res Commun. 2020;521:259-264 pubmed publisher
Sanyal A, Dutta S, Camara A, Chandran A, Koller A, Watson B, et al. Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson's Disease. J Mol Biol. 2019;431:2266-2282 pubmed publisher
Wördehoff M, Hoyer W. α-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay. Bio Protoc. 2018;8: pubmed publisher
Oliveira Souza W, Bronze F, Broos J, Marcondes M, Oliveira V. On the efficient bio-incorporation of 5-hydroxy-tryptophan in recombinant proteins expressed in Escherichia coli with T7 RNA polymerase-based vectors. Biochem Biophys Res Commun. 2017;492:343-348 pubmed publisher
Kumar S, Jangir D, Kumar R, Kumari M, Bhavesh N, Maiti T. Role of Sporadic Parkinson Disease Associated Mutations A18T and A29S in Enhanced ?-Synuclein Fibrillation and Cytotoxicity. ACS Chem Neurosci. 2018;9:230-240 pubmed publisher
Paleologou K, Schmid A, Rospigliosi C, Kim H, Lamberto G, Fredenburg R, et al. Phosphorylation at Ser-129 but not the phosphomimics S129E/D inhibits the fibrillation of alpha-synuclein. J Biol Chem. 2008;283:16895-905 pubmed publisher
product information
Catalog Number :
36046
Product Name :
pT7-7 asyn WT
article :
doi10.1074/jbc.M800747200
id5219
pubmed_id18343814
bacterial resistance :
Ampicillin
cloning :
backbonepT7-7
backbone_mutation
backbone_origin
backbone_size2438
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Lashuel Lab Plasmid #77
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoterT7
sequencing_primer_3AmpStop (TCAGGCAACTATGGATGAAC)
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutation
namealpha-synuclein
shRNA_sequence
size476
species
9606
Homo sapiens
tags
resistance markers :
1204
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA