pCDNA 3.1 Flag-rat-PTP1B | Addgene 35988 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA 3.1 Flag-rat-PTP1B

catalog :

35988

citations: 4

Reference
Li H, Li B, Larose L. IRE1? links Nck1 deficiency to attenuated PTP1B expression in HepG2 cells. Cell Signal. 2017;36:79-90 pubmed publisher
Wang X, Zheng R, Suo Z, Liu Y, Zhang Z, Ma Z, et al. Activity-dependent dephosphorylation of paxillin contributed to nociceptive plasticity in spinal cord dorsal horn. Pain. 2016;157:652-65 pubmed publisher
Chen P, Cai S, Yang Y, Li W, Huang C, Meng X, et al. PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells. Toxicol Appl Pharmacol. 2016;292:8-18 pubmed publisher
Cortesio C, Chan K, Perrin B, Burton N, Zhang S, Zhang Z, et al. Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion. J Cell Biol. 2008;180:957-71 pubmed publisher

product information

Catalog Number :

35988

Product Name :

pCDNA 3.1 Flag-rat-PTP1B

article :

doi	10.1083/jcb.200708048
id	5204
pubmed_id	18332219

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5427

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

and 5 -CTCTAGACTCGAGCGGCCGCTCAGTGAAAACATACCCGGTAACAG-3 . The PCR product was digested with BamHI and NotI and subsequently ligated into the corresponding sites of pCDNA3.1(+) (Invitrogen) Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 889-910, indicating that the NheI and AflII sites are no longer present in the plasmid.
-CTCGGATCCGCCACCATGGACTACAAGGACGACGATGA
CAAGAAGCTTGGGATGGAAATGGAGAAGGAATTCGAG-3
A Kozak methionine initiator sequence and a Flag tag were added to the N terminus of rat PTP1B, which was amplified by PCR with the following primers: 5

origin :

pi :

alt_names

PTP1B

cloning

clone_method	Restriction Enzyme
cloning_site_3	Not I
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGH Rev CCTCGACTGTGCCTTCTA
sequencing_primer_5	T7 FWD TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Ptp
gene	Ptpn1
id	24697

genbank_ids

mutation

name

protein tyrosine phosphatase 1B

shRNA_sequence

size

1299

species

10116

Rattus norvegicus