This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAC-4CL1
catalog :
35947
citations: 5
Reference
Rodrigues J, Gomes D, Rodrigues L. Challenges in the Heterologous Production of Furanocoumarins in Escherichia coli. Molecules. 2022;27: pubmed publisher
Rodrigues J, Gomes D, Rodrigues L. A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli. Front Bioeng Biotechnol. 2020;8:59 pubmed publisher
Couto M, Rodrigues J, Rodrigues L. Optimization of fermentation conditions for the production of curcumin by engineered Escherichia coli. J R Soc Interface. 2017;14: pubmed publisher
Afonso M, Ferreira S, Domingues F, Silva F. Resveratrol production in bioreactor: Assessment of cell physiological states and plasmid segregational stability. Biotechnol Rep (Amst). 2015;5:7-13 pubmed publisher
Watts K, Lee P, Schmidt Dannert C. Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006;6:22 pubmed
product information
Catalog Number :
35947
Product Name :
pAC-4CL1
article :
doi10.1186/1472-6750-6-22
id5192
pubmed_id16551366
bacterial resistance :
Chloramphenicol
cloning :
backbonepAC
backbone_mutation
backbone_origin
backbone_size4449
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth notes :
Initial gene cloning described in the following: Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783
origin :
37
pi :
alt_names
4CL
4CL1
cloning
clone_methodRestriction Enzyme
cloning_site_3NcoI
cloning_site_5NcoI
promoterpLac-mod
sequencing_primer_3GCA CAG ATG CGT AAG GAG AAA ATA CC
sequencing_primer_5TGT TGT GTG GAA TTG TGA GCG TCT AGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G51680, 4-COUMARATE:COA LIGASE, 4-COUMARATE:COA LIGASE 1, 4-coumarate:CoA ligase 1, 4CL.1, ARABIDOPSIS THALIANA 4-COUMARATE:COA LIGASE 1, AT4CL1, F19C24.11, F19C24_11
gene4CL1
id841593
genbank_ids
U18675.1
mutation
name4-coumaroyl CoA ligase 1
shRNA_sequence
size1722
species
3702
Arabidopsis thaliana
tags
resistance markers :
1066
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA