This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTWEEN-miR-224
catalog :
35683
product information
Catalog Number :
35683
Product Name :
pTWEEN-miR-224
article :
doi
id5178
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepRRL-CMV-PGK-GFP-WPRE (TWEEN)
backbone_mutation
backbone_originprovided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591)
backbone_size8600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP.
origin :
37
pi :
alt_names
MIR224
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoter
sequencing_primer_3GFP-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN224, miRNA224
geneMIR224
id407009
genbank_ids
407009
NC_000023.10
mutation
namensa-mir-224
shRNA_sequence
size561
species
9606
Homo sapiens
tags
resistance markers :
1131
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA