This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-PGK-KRAS4A(G12V)
catalog :
35634
citations: 3
Reference
Wong C, Wu J, Ji F, Kang W, Bian X, Chen H, et al. The cholesterol uptake regulator PCSK9 promotes and is a therapeutic target in APC/KRAS-mutant colorectal cancer. Nat Commun. 2022;13:3971 pubmed publisher
Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
Singh A, Sweeney M, Yu M, Burger A, Greninger P, Benes C, et al. TAK1 inhibition promotes apoptosis in KRAS-dependent colon cancers. Cell. 2012;148:639-50 pubmed publisher
product information
Catalog Number :
35634
Product Name :
pLenti-PGK-KRAS4A(G12V)
article :
doi10.1016/j.cell.2011.12.033
id5156
pubmed_id22341439
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-PGK-Hygro
backbone_mutation
backbone_originEric Campeau
backbone_size10000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
origin :
37
pi :
alt_names
KRAS4A
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3attL2
sequencing_primer_5attlL1
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationGlycine 12 to Valine
nameK-Ras4A(G12V)
shRNA_sequence
size550
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagV5
resistance markers :
1194
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA