This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-PGK-KRAS4B(G12V)
catalog :
35633
citations: 10
Reference
Collins S, Wiegand M, Werner A, Brown I, Mundo M, Swango D, et al. Ras-mediated activation of mTORC2 promotes breast epithelial cell migration and invasion. Mol Biol Cell. 2023;34:ar9 pubmed publisher
Suzuki T, Kishikawa T, Sato T, Takeda N, Sugiura Y, Seimiya T, et al. Mutant KRAS drives metabolic reprogramming and autophagic flux in premalignant pancreatic cells. Cancer Gene Ther. 2021;: pubmed publisher
Al Masri M, Paliotti K, Tran R, Halaoui R, Lelarge V, Chatterjee S, et al. Architectural control of metabolic plasticity in epithelial cancer cells. Commun Biol. 2021;4:371 pubmed publisher
Shorstova T, Su J, Zhao T, Dahabieh M, Leibovitch M, De Sa Tavares Russo M, et al. Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition. Mol Cancer Ther. 2021;20:64-75 pubmed publisher
Kharbanda A, Walter D, Gudiel A, Schek N, Feldser D, Witze E. Blocking EGFR palmitoylation suppresses PI3K signaling and mutant KRAS lung tumorigenesis. Sci Signal. 2020;13: pubmed publisher
Kharbanda A, Runkle K, Wang W, Witze E. Induced sensitivity to EGFR inhibitors is mediated by palmitoylated cysteine 1025 of EGFR and requires oncogenic Kras. Biochem Biophys Res Commun. 2017;493:213-219 pubmed publisher
Vaz M, Hwang S, Kagiampakis I, Phallen J, Patil A, O Hagan H, et al. Chronic Cigarette Smoke-Induced Epigenomic Changes Precede Sensitization of Bronchial Epithelial Cells to Single-Step Transformation by KRAS Mutations. Cancer Cell. 2017;32:360-376.e6 pubmed publisher
Zhang K, Myllymäki S, Gao P, Devarajan R, Kytölä V, Nykter M, et al. Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce anoikis resistance and synergizes with αV-Class integrins to promote EMT. Oncogene. 2017;36:5681-5694 pubmed publisher
de Jong P, Taniguchi K, Harris A, Bertin S, Takahashi N, Duong J, et al. ERK5 signalling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation. Nat Commun. 2016;7:11551 pubmed publisher
Singh A, Sweeney M, Yu M, Burger A, Greninger P, Benes C, et al. TAK1 inhibition promotes apoptosis in KRAS-dependent colon cancers. Cell. 2012;148:639-50 pubmed publisher
product information
Catalog Number :
35633
Product Name :
pLenti-PGK-KRAS4B(G12V)
article :
doi10.1016/j.cell.2011.12.033
id5156
pubmed_id22341439
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-PGK-Hygro
backbone_mutation
backbone_originEric Campeau
backbone_size10000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
origin :
37
pi :
alt_names
KRAS4B
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3attL2
sequencing_primer_5attL1
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationGlycine 12 to Valine
nameK-Ras4B(G12V)
shRNA_sequence
size550
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
1194
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA