This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3_NSlmb-vhhGFP4
catalog :
35579
citations: 16
Reference
Fletcher A, Clift D, de Vries E, Martinez Cuesta S, Malcolm T, Meghini F, et al. A TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation. Nat Commun. 2023;14:7093 pubmed publisher
Yu C, Runge H, Mukhopadhyay A, Zolles G, Ulbrich M. γ-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core. Cell Mol Biol Lett. 2023;28:54 pubmed publisher
Lin B, Luo J, Lehmann R. An AMPK phosphoregulated RhoGEF feedback loop tunes cortical flow-driven amoeboid migration in vivo. Sci Adv. 2022;8:eabo0323 pubmed publisher
Oliinyk O, Baloban M, Clark C, Carey E, Pletnev S, Nimmerjahn A, et al. Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies. Nat Methods. 2022;19:740-750 pubmed publisher
Fagbadebo F, Kaiser P, Zittlau K, Bartlick N, Wagner T, Froehlich T, et al. A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1. Front Mol Biosci. 2022;9:835302 pubmed publisher
Yu D, Do Heo W. Optogenetic Activation of Intracellular Nanobodies. Methods Mol Biol. 2022;2446:595-606 pubmed publisher
Lin B, Luo J, Lehmann R. Collectively stabilizing and orienting posterior migratory forces disperses cell clusters in vivo. Nat Commun. 2020;11:4477 pubmed publisher
Redchuk T, Karasev M, Verkhusha P, Donnelly S, Hülsemann M, Virtanen J, et al. Optogenetic regulation of endogenous proteins. Nat Commun. 2020;11:605 pubmed publisher
Yu D, Lee H, Hong J, Jung H, Jo Y, Oh B, et al. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods. 2019;16:1095-1100 pubmed publisher
Daniel K, Icha J, Horenburg C, Müller D, Norden C, Mansfeld J. Conditional control of fluorescent protein degradation by an auxin-dependent nanobody. Nat Commun. 2018;9:3297 pubmed publisher
Kanner S, Morgenstern T, Colecraft H. Sculpting ion channel functional expression with engineered ubiquitin ligases. elife. 2017;6: pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
Wang S, Tang N, Lara Gonzalez P, Zhao Z, Cheerambathur D, Prevo B, et al. A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development. 2017;144:2694-2701 pubmed publisher
Park H, Lee S, Heo W. Protein Inactivation by Optogenetic Trapping in Living Cells. Methods Mol Biol. 2016;1408:363-76 pubmed publisher
Stewart Ornstein J, Lahav G. Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Cell Rep. 2016;14:1800-1811 pubmed publisher
Caussinus E, Kanca O, Affolter M. Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nat Struct Mol Biol. 2011;19:117-21 pubmed publisher
product information
Catalog Number :
35579
Product Name :
pcDNA3_NSlmb-vhhGFP4
article :
doi10.1038/nsmb.2180
id5152
pubmed_id22157958
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
slmb
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3atttaggtgacactatag
sequencing_primer_5taatacgactcactataggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG3412, BcDNA:GM02031, CG3412, Dmel CG3412, FBXW1, MENE (3R)-B, MENE(3R)-B, SLIMB, SLMB, Slimb, Slimb/Beta-TRCP1, Slimb/beta-TRCP, Slimb/beta-TrCP, Slimb/betaTrCP, Slmb, beta-TrCP, betaTrCP, crd, ica, l(3)00295, shv, slimb, wel
geneslmb
id42504
genbank_ids
AY118898
mutation
nameNSlmb-vhhGFP4
shRNA_sequence
size951
species
7227
Drosophila melanogaster
tags
resistance markers :
1185
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA