This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MS2-HB
catalog :
35573
citations: 5
Reference
Delhaye L, De Bruycker E, Volders P, Fijalkowska D, De Sutter D, Degroeve S, et al. Orthogonal proteomics methods to unravel the HOTAIR interactome. Sci Rep. 2022;12:1513 pubmed publisher
Lv T, Jin Y, Miao Y, Xu T, Jia F, Feng H, et al. LncRNA PVT1 promotes tumorigenesis of glioblastoma by recruiting COPS5 to deubiquitinate and stabilize TRIM24. Mol Ther Nucleic Acids. 2022;27:109-121 pubmed publisher
Tang J, Yu B, Li Y, Zhang W, Alvarez A, Hu B, et al. TGF-β-activated lncRNA LINC00115 is a critical regulator of glioma stem-like cell tumorigenicity. EMBO Rep. 2019;20:e48170 pubmed publisher
Ade C, Derbes R, Wagstaff B, Linker S, White T, Deharo D, et al. Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Gene. 2018;642:188-198 pubmed publisher
Tsai B, Wang X, Huang L, Waterman M. Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Mol Cell Proteomics. 2011;10:M110.007385 pubmed publisher
product information
Catalog Number :
35573
Product Name :
MS2-HB
article :
doi10.1074/mcp.M110.007385
id5151
pubmed_id21285413
bacterial resistance :
Ampicillin
cloning :
backbonepQCXIP
backbone_mutation
backbone_originQiagen
backbone_size7480
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
origin :
37
pi :
alt_names
N55K
cloning
clone_methodRestriction Enzyme
cloning_site_3Pac1
cloning_site_5Not1
promoterCMV
sequencing_primer_3IRES-R
sequencing_primer_5LNCX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMS2g2
genecp
id1260899
genbank_ids
mutationInsert is a tail-to-head tandem placement of two MS2 coat protein open reading frames. Both MS2 contains a N55K mutation that increases protein affinity to MS2 stem loop
nameMS2 Coat Protein
shRNA_sequence
size777
species
Bacteriophage
tags
locationC terminal on backbone
tagHTBH
plasmid copy :
MS2 coat protein sequence was amplified from pCT119-N55K (gift from David Peabody, University of New Mexico).
resistance markers :
1132
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA