This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tagged-IRES
catalog :
35570
citations: 3
Reference
Francisco Velilla R, Fernandez Chamorro J, Dotu I, Martinez Salas E. The landscape of the non-canonical RNA-binding site of Gemin5 unveils a feedback loop counteracting the negative effect on translation. Nucleic Acids Res. 2018;46:7339-7353 pubmed publisher
Lozano G, Francisco Velilla R, Martinez Salas E. Ribosome-dependent conformational flexibility changes and RNA dynamics of IRES domains revealed by differential SHAPE. Sci Rep. 2018;8:5545 pubmed publisher
Tsai B, Wang X, Huang L, Waterman M. Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Mol Cell Proteomics. 2011;10:M110.007385 pubmed publisher
product information
Catalog Number :
35570
Product Name :
Tagged-IRES
article :
doi10.1074/mcp.M110.007385
id5151
pubmed_id21285413
bacterial resistance :
Ampicillin
cloning :
backbonepRSTF
backbone_mutationRenilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
backbone_originJang et al. 2004
backbone_size4660
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
origin :
37
pi :
alt_names
LEF1 IRES
cloning
clone_methodRestriction Enzyme
cloning_site_3Afe1
cloning_site_5Afe1
promoterSV40
sequencing_primer_3LucNrev
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesLEF-1, TCF10, TCF1ALPHA, TCF7L3
geneLEF1
id51176
genbank_ids
mutation
nameLymphoid Enhancer Factor 1 5'UTR
shRNA_sequence
size1183
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFirefly Luciferase
locationC terminal on backbone
tagMS2 Stem Loop
resistance markers :
1132
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA