This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL3-1574/+120
catalog :
35539
citations: 2
Reference
Lu Z, Jiao D, Qiao J, Yang S, Yan M, Cui S, et al. Restin suppressed epithelial-mesenchymal transition and tumor metastasis in breast cancer cells through upregulating mir-200a/b expression via association with p73. Mol Cancer. 2015;14:102 pubmed publisher
Bracken C, Gregory P, Kolesnikoff N, Bert A, Wang J, Shannon M, et al. A double-negative feedback loop between ZEB1-SIP1 and the microRNA-200 family regulates epithelial-mesenchymal transition. Cancer Res. 2008;68:7846-54 pubmed publisher
product information
Catalog Number :
35539
Product Name :
pGL3-1574/+120
article :
doi10.1158/0008-5472.CAN-08-1942
id5146
pubmed_id18829540
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-basic
backbone_mutation
backbone_originPromega
backbone_size4818
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
Please note that Addgene's sequencing results identified a few nucleotide mismatches at bp# 253, 457, 465, 632, 681 & 1463 when compared to the sequence provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.
origin :
37
pi :
alt_names
MIRN200B
MIRN200A
MIRN429
cloning
clone_methodRestriction Enzyme
cloning_site_3BglI
cloning_site_5NheI
promoternone
sequencing_primer_3CTTTATGTTTTTGGCGTCTTCCA
sequencing_primer_5-CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN200B, mir-200b
geneMIR200B
id406984
aliasesMIRN429, hsa-mir-429, mir-429
geneMIR429
id554210
aliasesMIRN200A, mir-200a
geneMIR200A
id406983
genbank_ids
406984
406983
mutation
namepromoter region of miR200b-200a-429 genomic cluster
shRNA_sequence
size1694
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagluciferase
resistance markers :
1220
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA