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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3/Myc-DNMT3A
catalog :
35521
citations: 30
Reference
Pathak H, Borchert A, Garaali S, Burkert A, Frieling H. BDNF exon IV promoter methylation and antidepressant action: a complex interplay. Clin Epigenetics. 2022;14:187 pubmed publisher
Singh K, Rustagi Y, Abouhashem A, Tabasum S, Verma P, Hernandez E, et al. Genome-wide DNA hypermethylation opposes healing in patients with chronic wounds by impairing epithelial-mesenchymal transition. J Clin Invest. 2022;132: pubmed publisher
Lu J, Zhen S, Tuo X, Chang S, Yang X, Zhou Y, et al. Downregulation of DNMT3A Attenuates the Warburg Effect, Proliferation, and Invasion via Promoting the Inhibition of miR-603 on HK2 in Ovarian Cancer. Technol Cancer Res Treat. 2022;21:15330338221110668 pubmed publisher
Huang X, Zhang X, Zong L, Gao Q, Zhang C, Wei R, et al. Gene body methylation safeguards ribosomal DNA transcription by preventing PHF6-mediated enrichment of repressive histone mark H4K20me3. J Biol Chem. 2021;297:101195 pubmed publisher
Sidler M, Aitken K, Jiang J, Yadav P, Lloyd E, Ibrahim M, et al. Inhibition of DNA methylation during chronic obstructive bladder disease (COBD) improves function, pathology and expression. Sci Rep. 2021;11:17307 pubmed publisher
Kaplun D, Starshin A, Sharko F, Gainova K, Filonova G, Zhigalova N, et al. Kaiso Regulates DNA Methylation Homeostasis. Int J Mol Sci. 2021;22: pubmed publisher
Zhang X, Zhao S, Yuan Q, Zhu L, Li F, Wang H, et al. TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression. Cell Death Dis. 2021;12:642 pubmed publisher
Noyan S, Andac Ozketen A, Gurdal H, Gur Dedeoglu B. miR-770-5p regulates EMT and invasion in TNBC cells by targeting DNMT3A. Cell Signal. 2021;83:109996 pubmed publisher
Masalmeh R, Taglini F, Rubio Ramon C, Musialik K, Higham J, Davidson Smith H, et al. De novo DNA methyltransferase activity in colorectal cancer is directed towards H3K36me3 marked CpG islands. Nat Commun. 2021;12:694 pubmed publisher
Han X, Liu D, Zhou Y, Wang L, Hou H, Chen H, et al. The negative feedback between miR-143 and DNMT3A regulates cisplatin resistance in ovarian cancer. Cell Biol Int. 2021;45:227-237 pubmed publisher
González Rodríguez P, Cheray M, Füllgrabe J, Salli M, Engskog Vlachos P, Keane L, et al. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy. 2020;:1-19 pubmed publisher
Bayraktar G, Yuanxiang P, Confettura A, Gomes G, Raza S, Stork O, et al. Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus. Neuropsychopharmacology. 2020;45:2120-2130 pubmed publisher
Liu W, Irudayaraj J. Perfluorooctanoic acid (PFOA) exposure inhibits DNA methyltransferase activities and alters constitutive heterochromatin organization. Food Chem Toxicol. 2020;141:111358 pubmed publisher
Lu J, Chen H, He F, You Y, Feng Z, Chen W, et al. Ginsenoside 20(S)-Rg3 upregulates HIF-1α-targeting miR-519a-5p to inhibit the Warburg effect in ovarian cancer cells. Clin Exp Pharmacol Physiol. 2020;47:1455-1463 pubmed publisher
Finnegan A, Kim S, Jin H, Gapinske M, Woods W, Perez Pinera P, et al. Epigenetic engineering of yeast reveals dynamic molecular adaptation to methylation stress and genetic modulators of specific DNMT3 family members. Nucleic Acids Res. 2020;48:4081-4099 pubmed publisher
Brezgin S, Kostyusheva A, Bayurova E, Gordeychuk I, Isaguliants M, Goptar I, et al. Replenishment of Hepatitis B Virus cccDNA Pool Is Restricted by Baseline Expression of Host Restriction Factors In Vitro. Microorganisms. 2019;7: pubmed publisher
Lyu G, Zong L, Zhang C, Huang X, Xie W, Fang J, et al. Metastasis-related methyltransferase 1 (Merm1) represses the methyltransferase activity of Dnmt3a and facilitates RNA polymerase I transcriptional elongation. J Mol Cell Biol. 2018;: pubmed publisher
Kim G, Kwon H, Lee C, Verma R, Rudra D, Kim T, et al. Upregulation of Ets1 expression by NFATc2 and NFKB1/RELA promotes breast cancer cell invasiveness. Oncogenesis. 2018;7:91 pubmed publisher
Huang M, Chou Y, Li M, Shih T, Lin S, Chuang H, et al. Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition. Pharmacol Res. 2019;139:50-61 pubmed publisher
Lu J, Wang L, Chen W, Wang Y, Zhen S, Chen H, et al. miR-603 targeted hexokinase-2 to inhibit the malignancy of ovarian cancer cells. Arch Biochem Biophys. 2019;661:1-9 pubmed publisher
Dhar S, Zhao D, Lin T, Gu B, Pal K, Wu S, et al. MLL4 Is Required to Maintain Broad H3K4me3 Peaks and Super-Enhancers at Tumor Suppressor Genes. Mol Cell. 2018;70:825-841.e6 pubmed publisher
Lin L, Liu Y, Xu F, Huang J, Daugaard T, Petersen T, et al. Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. Gigascience. 2018;7:1-19 pubmed publisher
Zhou Y, Zheng X, Lu J, Chen W, Li X, Zhao L. Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells. Cell Physiol Biochem. 2018;45:2548-2559 pubmed publisher
Cui Y, Choudhury S, Irudayaraj J. Epigenetic Toxicity of Trichloroethylene: A Single-Molecule Perspective. Toxicol Res (Camb). 2016;5:641-650 pubmed publisher
Li J, Lu J, Ye Z, Han X, Zheng X, Hou H, et al. 20(S)-Rg3 blocked epithelial-mesenchymal transition through DNMT3A/miR-145/FSCN1 in ovarian cancer. Oncotarget. 2017;8:53375-53386 pubmed publisher
Liu X, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, et al. Editing DNA Methylation in the Mammalian Genome. Cell. 2016;167:233-247.e17 pubmed publisher
Choudhury S, Cui Y, Narayanan A, Gilley D, Huda N, Lo C, et al. Optogenetic regulation of site-specific subtelomeric DNA methylation. Oncotarget. 2016;7:50380-50391 pubmed publisher
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Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016;44:5615-28 pubmed publisher
Chen Z, Mann J, Hsieh C, Riggs A, Chedin F. Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family. J Cell Biochem. 2005;95:902-17 pubmed
product information
Catalog Number :
35521
Product Name :
pcDNA3/Myc-DNMT3A
article :
doi10.1002/jcb.20447
id5141
pubmed_id15861382
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationpcDNA3/Myc was constructed by Zhaoxia Chen (see Comments section for details). DNMT1 was cloned into the pcDNA3/Myc vector.
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT3A was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen
growth strain :
Mammalian expression of human DNMT3A with myc tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
AF067972
mutation
nameDNMT3A
shRNA_sequence
size2739
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyc
resistance markers :
1181
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA